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目的探讨不同类型胃息肉中微卫星不稳定(MSI)的变化。方法选取中国医科大学附属一院内镜中心和肿瘤标本,其中炎性息肉、增生性息肉、腺瘤型息肉、胃腺癌每组各30例,通过饱和氯化钠法提取组织DNA,通过PCR-SSCP变性聚丙烯酰胺凝胶电泳检测MSI。结果 MSI在炎性息肉、增生性息肉,腺瘤型息肉,胃腺癌中的检出率分别为13.3%、16.7%、30%、40%,腺瘤型息肉与胃腺癌有统计学差异(P<0.05)。结论 MSI是胃癌发生中的早期分子标志,MSI可以作为息肉恶变的早期预测因子。
Objective To investigate the changes of microsatellite instability (MSI) in different types of gastric polyp. Methods The endoscopic centers and tumor specimens from the First Affiliated Hospital of China Medical University were selected. Thirty cases of inflammatory polyps, hyperplastic polyps, adenomatous polyps and gastric adenocarcinoma were collected from each group. The tissue DNA was extracted by saturated sodium chloride solution. SSCP denaturing polyacrylamide gel electrophoresis detection of MSI. Results The positive rates of MSI were 13.3%, 16.7%, 30% and 40% respectively in adenocarcinoma, adenomatous polyp, adenomatous polyp, adenomatous polyp and gastric adenocarcinoma <0.05). Conclusion MSI is an early molecular marker of gastric carcinogenesis, MSI can be used as an early predictor of polypoid malignancy.