格兰氏染色是植物病原细菌分类根据之一。进行常规的格兰氏染色,必须配制染色剂,在染色过程中如酒精褪色掌握不当,染色反应容易混淆。据国外文献介绍,将细菌移在金氏培养基B(KB agar)上培养后,用氢氧化钾水溶液处理,就可以区别格兰氏阴性菌和阳性菌。根据以上方法,用六种植物原细菌和枯草杆菌进行试验,反应明确。82—83年在教学和科研上应用,收到良好效果。一、材料和方法 Gram stain is one of the classification of plant pathogenic bacteria. For conventional Gram staining, it is necessary to formulate coloring agents, such as alcohol fading mishandling in the dyeing process, and staining reactions are easily confused. According to foreign literature, the bacteria were cultured in KB medium (B agar) cultured with potassium hydroxide solution, you can distinguish between Gram-negative bacteria and positive bacteria. According to the above method, six plant protozoa and Bacillus subtilis were tested and the reaction was clear. 82-83 years of teaching and research applications, received good results. First, materials and methods