选择８只日本大耳白兔，移动下颌第一磨牙向近中方向，实验期限为２周。在动物处死取材进行组织学研究时，采用了硬组织磨片法以及特殊的染色技术，观察了正畸牙齿移动过程中新骨生成的情况。结果证明，该研究方法有以下优越性，新骨染色与原骨容易辩认；观察直观；组织保存完整而不易变形；其表现与普通组织学相一致。 Choose eight Japanese white rabbits, move the first molar of the mandible to the near direction, the experimental period of 2 weeks. When the animals were sacrificed for histological study, hard tissue grinding method and special staining technique were used to observe the new bone formation during orthodontic tooth movement. The results show that the research methods have the following advantages, easy to identify the new bone staining and bone; intuitive observation; complete preservation of the organization is not easy to deform; its performance consistent with the general histology.