论文部分内容阅读
背景:通过竞争性抑制一氧化氮合酶(nitricoxidesynthase,NOS),可损害大鼠的学习记忆行为,表明NO/NOS对维持正常的学习记忆功能是必需的,但有关学习记忆障碍时NOS神经元的变化尚不十分清楚。目的:探讨NOS神经元与学习记忆障碍的关系。设计:完全随机设计,对照实验研究。地点和材料:本研究地点为中南大学湘雅医学院人体解剖学和神经生物学系,材料为健康昆明雄性小鼠32只,6～8周龄,体质量20～25g,购于湖南医科大学动物学部。干预:32只小鼠随机分为模型组和对照组,每组各取7d和14d两个时间点,每个时间点各8只小鼠。采用辐射式三等份Y型迷宫测试装置,检测动物的空间辨别性学习记忆能力。应用免疫组化结合组织化学方法显示学习记忆障碍小鼠脑中NOS神经元的变化。主要观察指标:检测动物的空间辨别性学习记忆能力,观察切片NOS神经元形态并计数。结果:随训练次数的增加,对照组小鼠在Y型迷宫中的正确次数逐渐增加(术前第1天8.3±1.2,术后第7天10.1±1.0),而模型组小鼠术后在Y型迷宫测试中的正确次数较术前(8.3±1.2)明显减少(术后第7天4.7±2.4,P<0.05),与对照组同时间点相比,差异有显著性意义(P<0.01)。但术后14d模型组小鼠在迷宫测试中的正确次数渐有增加(9.3±0.7)。学习记忆障碍小鼠海马CA1-4区NOS神经元数目显
BACKGROUND: Competitive inhibition of nitric oxide synthase (NOS) can impair the learning and memory behavior of rats, indicating that NO / NOS is essential for maintaining normal learning and memory. However, NOS neurons The changes are not very clear. Objective: To investigate the relationship between NOS neurons and learning and memory impairment. Design: Completely randomized design, controlled experimental study. Location and Materials: The study was conducted at Xiangya Medical College of Central South University Department of Human Anatomy and Neurobiology, the material is healthy Kunming male mice 32, 6 to 8 weeks of age, body weight 20 ~ 25g, purchased from Hunan Medical University Department of Zoology Intervention: 32 mice were randomly divided into model group and control group. Each group took two time points of 7d and 14d, each of 8 mice at each time point. Radiation three equal parts Y-type maze test device, the detection of animal spatial learning and memory ability. Immunohistochemistry and histochemistry were used to show the changes of NOS neurons in the brain of learning and memory deficient mice. MAIN OUTCOME MEASURES: The animals were tested for spatial discriminative learning and memory ability, and morphological changes of NOS neurons were observed and counted. Results: With the increase of training times, the correct number of mice in the control group increased gradually in the Y-maze (8.3 ± 1.2 on the first day before surgery and 10.1 ± 1.0 on the seventh day after the operation) The correct number of Y-maze test was significantly lower than that of the preoperative (8.3 ± 1.2) (4.7 ± 2.4, P <0.05 on the 7th day after operation), which was significantly different from that of the control group at the same time point (P < 0.01). However, the correct number of mice in the model group at the 14th day after operation in the maze test gradually increased (9.3 ± 0.7). The number of NOS neurons in hippocampal CA1-4 area of mice with learning and memory impairment