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目的探讨乙型脑炎病毒反向遗传学系统在临床中的应用价值。方法对在我院自2011年l2月至2013年1月接受治疗的80例乙型脑炎病毒患者资料进行回顾性分析,医护人员根据患者住院时间将其分为实验组和对照组,每组有患儿40名。对照组患者采用传统方法进行治疗,实验组患者在传统方法基础上利用分子生物学技术构建获得乙型脑炎病毒复制子,比较两组患者的诊断效果及其价值。结果实验组患者总有效率为95%,优于对照组患者;两组患者神经功能状况指数、患者意识评分等指标治疗前没有差异性,患者治疗后,两组患者指标明显改善,实验组患者改善效果更好FEV1(87.2±10.5VS70.1±8.2)。结论临床上医护人员对乙型脑炎病毒在常规诊断和治疗基础上利用反向遗传学技术临床上效果较好,患者确诊率较高,能够为治疗乙型脑炎病毒提供依据,值得推广使用。
Objective To investigate the clinical value of reverse genetics system of Japanese encephalitis virus (JEV) in clinical practice. Methods The data of 80 patients with Japanese encephalitis virus treated in our hospital from January 2011 to January 2013 were retrospectively analyzed. The medical staff were divided into experimental group and control group according to hospitalization time, and each group There are 40 children. Patients in the control group were treated by traditional methods. Patients in the experimental group were screened by molecular biology techniques to obtain JEV replicons based on the traditional methods. The diagnostic value and the value of the two groups of patients were compared. Results The total effective rate of the experimental group was 95%, which was better than that of the control group. There was no difference between the two groups before and after the treatment of neurological status index and patient’s consciousness score. After treatment, the indexes of the two groups were significantly improved. Better improvement of FEV1 (87.2 ± 10.5VS70.1 ± 8.2). Conclusions Clinicians in clinical use of reverse genetics based on routine diagnosis and treatment of Japanese encephalitis virus have a good clinical effect and a high diagnosis rate of patients with JE, which may provide a basis for treatment of Japanese Encephalitis virus and should be promoted .