目的:研究辅助生殖治疗的多囊卵巢综合征(PCOS)患者卵泡中干细胞因子(SCF)、生长分化因子-9(GDF-9)和骨形态发生蛋白-15(BMP-15)的表达水平与卵泡发育异常的关系。方法:收集30例PCOS患者和40例正常对照者的卵泡液和颗粒细胞。采用酶联免疫吸附实验(ELISA)检测卵泡液中SCF、GDF-9和BMP-15的表达水平,采用免疫荧光染色法测定颗粒细胞中SCF的表达水平。结果:PCOS患者(n=30)卵泡液中GDF-9、BMP-15和SCF的浓度均明显低于正常对照组(n=40),差异有统计学意义(P<0.05)。与对照组相比,PCOS患者颗粒细胞中SCF的表达水平显著下降(P<0.05)。结论:PCOS患者卵泡液中GDF-9、BMP-15和SCF的表达均明显降低,并且PCOS患者颗粒细胞中SCF的表达也显著下降,这可能与其卵泡发育不良有关。 OBJECTIVE: To investigate the expression of follicular stem cell factor (SCF), growth differentiation factor-9 (GDF-9) and bone morphogenetic protein-15 (BMP-15) in women with polycystic ovary syndrome (PCOS) assisted reproductive therapy Follicular dysplasia relationship. Methods: Follicular fluid and granulosa cells from 30 PCOS patients and 40 normal controls were collected. The expression of SCF, GDF-9 and BMP-15 in follicular fluid was detected by enzyme linked immunosorbent assay (ELISA), and the expression of SCF in granulosa cells was detected by immunofluorescence staining. Results: The concentrations of GDF-9, BMP-15 and SCF in follicular fluid of PCOS patients (n = 30) were significantly lower than those of normal control group (n = 40) (P <0.05). Compared with the control group, the expression of SCF in PCOS patients was significantly decreased (P <0.05). CONCLUSION: The expression of GDF-9, BMP-15 and SCF in follicular fluid of patients with PCOS were significantly decreased, and the expression of SCF in granulosa cells of PCOS patients was also significantly decreased, which may be related to follicular dysplasia.