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为探讨蛇毒抗菌肽OH-CATH对大肠杆菌标准株和临床耐药株引起家兔泌尿系感染的保护作用,该文利用大肠杆菌标准株(E.coli ATCC25922)和耐头孢菌素大肠杆菌临床耐药株建立雄性家兔泌尿系感染模型。通过膀胱直接给药的方式,分别给予动物感染模型生理盐水、蛇毒抗菌肽OH-CATH及头孢哌酮舒巴坦,并于给药后第1、5、10及14天留取家兔中段尿用于尿液培养。将动物膀胱标本行H-E染色石蜡切片及透射电镜观察。结果显示:使用蛇毒抗菌肽OH-CATH的大肠杆菌标准株和临床耐药株不同组别中段尿培养阳性率明显降低;给予头孢哌酮舒巴坦可降低大肠杆菌标准株(E.coli ATCC25922)引起感染的阳性率,但对耐头孢菌素大肠杆菌引起的感染的阳性率则无明显作用(P<0.05);使用蛇毒抗菌肽OH-CATH组炎症细胞浸润、坏死及钙化组织较其他组为少。该结果提示蛇毒抗菌肽OH-CATH对大肠杆菌标准株(E.coli ATCC25922)及耐头孢菌素大肠埃希菌临床耐药株有稳定活性,对其引起的家兔泌尿系感染有较好的保护作用,并为临床日益严重的耐药病原菌感染的治疗提供了新的思路和方向。
To investigate the protective effect of OH-CATH, an antimicrobial peptide from snake venom, on the urinary tract infection in rabbits induced by Escherichia coli standard strains and clinical drug-resistant strains, the clinical isolates of E. coli ATCC25922 and Cephalosporin-resistant Escherichia coli Establishment of a model of urinary tract infection in male rabbits. Animals were infected with saline, snake venom antibacterial peptide OH-CATH and cefoperazone sulbactam, respectively. The rabbits were sacrificed on days 1, 5, 10 and 14 For urine culture. Animal bladder specimens were examined by H-E staining and transmission electron microscopy. The results showed that the positive rate of urinary culture in different groups of standard strains and clinical drug-resistant strains of OH-CATH with anti-bacterial peptide OH-CATH significantly decreased; cefoperazone sulbactam could reduce the standard strain of E. coli (ATCC25922) (P <0.05). The percentage of inflammatory cell infiltration, necrosis and calcification in OH-CATH group was significantly higher than that in other groups less. The results suggest that OH-CATH has stable activity against E. coli ATCC25922 and Cephalosporium-resistant Escherichia coli strains, and has good urinary tract infection in rabbits Protection and provide a new way of thinking and direction for the treatment of increasingly serious clinical drug-resistant pathogen infections.