Background:Tuberculosis (TB) remains a worldwide problem.Intestinal TB (ITB) constitutes a major public health problem in developing countries and has been associated with significant morbidity and mortality.The aim of this study was to characterize the clinical,radiological,endoscopic,and pathological features of ITB and to define the strategy for establishing the diagnosis.Methods:A retrospective study (from January 2000 to June 2015) was carried out in Peking Union Medical College Hospital and all hospitalized cases were diagnosed as ITB during the study period were included.The relevant clinical information,laboratory results,microbiological,and radiological investigations were recorded.Results:Of the 85 cases,61 cases (71.8％) were ranged from 20 to 50 years.The ileocecal region was involved in about 83.5％ (71/85) of patients.About 41.2％ (35/85) of patients had co-existing extra ITB,especially active pulmonary TB.Abdominal pain (82.4％) was the most common presenting symptom followed by weight loss (72.9％) and fever (64.7％).Both T-cell spot of TB test (T-SPOT.TB) and purified protein derivatives (PPD) tests were performed in 26 patients:20 (76.9％) positive T-SPOT.TB and 13 (50.0％) positive PPD were detected,with a statistical significant difference (P =0.046).Twenty cases (23.5％) were histopathology and/or pathogen confirmed TB;27 cases (31.8％) were diagnosed by clinical manifestation consistent with ITB and evidence of active extra ITB;38 cases (44.7％) were diagnosed by good response to diagnostic anti-TB therapy.Conclusions:ITB is difficult to diagnose even with modem medical techniques due to its nonspecific clinical and laboratory features.At present,combination of clinical,endoscopic,radiological,and pathological features continues to be the key to the diagnosis of ITB.