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目的 观察高压氧 (HBO)对机体免疫功能的影响及可能机制。方法 小鼠 10只暴露于 0 .2 5MPa 99.2 %O2 的高压氧下 2h ,测定脾脏、胸腺淋巴细胞免疫粘附功能、丙二醛 (MDA)含量、血浆β -内啡肽 (β -EP)浓度。同时 ,小鼠各 10只注射SOD、维生素E及纳洛酮后观察上述指标变化。结果 HBO作用下淋巴细胞免疫功能显著下降 ,而MDA含量及血浆 β -EP浓度升高。应用SOD、维生素E可使MDA含量及血浆 β -EP浓度下降 ,抗氧化剂及纳洛酮均可使淋巴细胞粘附功能部分恢复。结论 HBO环境下 ,免疫器官的氧化损伤及血浆 β -EP浓度的升高对淋巴细胞免疫粘附功能有一定的抑制作用
Objective To observe the effect of hyperbaric oxygen (HBO) on immune function and its possible mechanism. Methods 10 mice were exposed to 0,25 MPa 99.2% O2 for 2 hours. The immune function, MDA content, β - endorphin (β - EP) concentration. Meanwhile, mice were injected with SOD, vitamin E and naloxone 10 respectively. Results Under the action of HBO, the immune function of lymphocytes decreased significantly, while the content of MDA and the concentration of plasma β-EP increased. Application of SOD, vitamin E can make the MDA content and plasma β-EP concentration decreased, antioxidants and naloxone can lymphocyte adhesion function partially restored. Conclusion Under the condition of HBO, the oxidative damage of immune organs and the increase of plasma β-EP concentration may have certain inhibitory effect on the immune function of lymphocytes