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目的观察慢性乙型肝炎重叠ＨＧＶ感染对ＨＢＶ复制的影响．方法采用逆转录聚合酶链反应（ＲＴ－ＰＣＲ）检测患者血清ＨＢＶ－ＤＮＡ．ＨＢＶ－ＤＮＡ定量采用荧光信号引物能量转换法．以单纯慢性乙型肝炎患者作对照研究．结果２３例ＨＧＶ重叠感染的慢性乙型肝炎患者 ＨＢＶ－ＤＮＡ、ＨＢｅＡｇ和抗－ＨＢｅ的阳性率分别为５６．５％、１７．４％和６０．９％而３４例单纯慢性乙型肝炎患者三项指标的阳性率分别为９１．２％、８５．３％和１１．８％．定量分析显示，前组ＨＢＶ－ＤＮＡ量为２．０２、后组为４．１２．经统计学处理，两组各项指标均有显著性差异．结论慢性乙型肝炎重叠ＨＧＶ感染可能会抑制ＨＢＶ的复制．
Objective To observe the effect of overlapping HGV infection on HBV replication in chronic hepatitis B patients. Methods Serum HBV-DNA was detected by reverse transcription-polymerase chain reaction (RT-PCR). HBV-DNA quantification using fluorescent signal primer energy conversion method. Chronic hepatitis B patients as a control study. Results The positive rates of HBV-DNA, HBeAg and anti-HBe in 23 patients with HGV overlap infection were 56.5%, 17.4% and 60.9% respectively, while those in 34 patients with chronic hepatitis B The positive rates of the indicators were 91.2%, 85.3% and 11.8% respectively. Quantitative analysis showed that the amount of HBV-DNA in the former group was 2.02 and that in the latter group was 4.12. After statistical analysis, the two groups of indicators have significant differences. Conclusions HGV infection in patients with chronic hepatitis B may inhibit HBV replication.