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目的研究醛固酮介导一肾一夹肾性高血压大鼠脑、肾、脾、肺、主动脉和肝脏组织钙调神经磷酸酶(CaN)活性的变化。方法取20只Wistar 大鼠随机分为3组:假手术组(sham op, n=6),一肾一夹手术组(op,n=7)和螺内酯干预组(spiro,n=7)。以PNPP为底物测定术后4周大鼠各组织器官CaN活性。结果肾性高血压大鼠脾、肾脏、肺、脑CaN活性较假手术组分别上升90%,84%,57%和43%(均P<0.05),螺内酯干预组上述组织器官CaN活性较手术组明显下降(P<0.05)。肾性高血压大鼠组肝脏CaN活性较假手术组下降27%(P<0.05),螺内酯干预组肝细胞CaN活性较手术组有下降趋势,但差异无显著意义。各组大鼠主动脉中CaN活性无明显变化。结论醛固酮对肾性高血压相关靶器官—脑、肾、脾、肺组织细胞内信号分子CaN的激活具有重要作用。
Objective To investigate the changes of calcineurin (CaN) activity induced by aldosterone in the brain, kidney, spleen, lung, aorta and liver of one-kidney-one-clip hypertensive rats. Methods Twenty Wistar rats were randomly divided into three groups: sham op group (n = 6), sham ophthalmic group (op n = 7) and spiro group (n = 7). PNPP was used as substrate to measure the activity of CaN in tissues and organs of rats after 4 weeks. Results The activity of CaN in spleen, kidney, lung and brain of renal hypertensive rats increased by 90%, 84%, 57% and 43% respectively (all P <0.05), and the activity of CaN in the splenic, Group decreased significantly (P <0.05). The hepatic CaN activity in renal hypertensive rats decreased by 27% (P <0.05) compared with that in sham operation group. The activity of CaN in hepatocytes in spironolactone intervention group decreased compared with that in operation group, but the difference was not significant. There was no significant change in CaN activity in the aorta of rats in each group. Conclusion Aldosterone plays an important role in the activation of intracellular signaling molecule CaN in target organs of kidney - related hypertension such as brain, kidney, spleen and lung.