现代免疫学中免疫染色、免疫杂交和单克隆抗体技术已广泛应用于内耳发育与功能方面的基础研究以及一些病理过程的特殊研究,如遗传性聋和特发性进行性双耳感音神经性聋(自身免疫疾病)。这些技术都需要内耳组织作为抗原提取剂。人内耳组织虽然是很理想的材料,但其来源困难且供应量不足。典型的实验哺乳动物如兔、鼠、猫或豚鼠虽然可以选用,但每一个小动物提供的组织量也有限。为了获得适用和足量的膜性内耳组织,人们开始采用牛颞骨内耳组织。该文报道获取和解剖牛颞骨的技术及牛内耳抗原制备与保存方法。研究表明采用人尸体内耳组织作为抗原源有两点不足,即内耳组织自溶及取量不足。基于后一原因须对每一新标本反复进行抗原成 Modern immunology immunostaining, immunization and monoclonal antibody technology has been widely used in the development and function of the inner ear, basic research and some special studies of pathological processes, such as hereditary deafness and idiopathic progressive binaural sensorineural Deaf (autoimmune disease). These techniques require inner ear tissue as an antigen extractor. Although the human inner ear tissue is a very ideal material, its source is difficult and the supply is insufficient. Although typical experimental mammals such as rabbits, mice, cats or guinea pigs are available, each animal provides only a limited amount of tissue. In order to obtain a suitable and sufficient amount of membranous inner ear tissue, bovine temporal bone and inner ear tissue has been used. This article reports the techniques of obtaining and dissecting bovine temporal bone and the preparation and preservation of bovine inner ear antigen. Studies have shown that using human ear tissue as an antigen source has less than two points, namely autologous inner ear tissue and insufficient amount. For each new sample, the antigen should be repeated for the latter reason