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目的:探讨急性重复性缺氧时脑氨基酸和第二信使变化的意义。方法:将72只小白鼠随机分为4组。Ⅰ组为盐水对照组,Ⅱ组为盐水适应组,Ⅲ组为给药适应组,Ⅳ组为给药对照组。Ⅲ组及Ⅳ组又被各分成3个亚组,分别腹腔注入氨氧乙酸25mg/kg,羟基丁酸钠60mg/kg及3-巯基丙酸20μg/kg。采用反相高效液相色谱检测各组脑氨基酸含量、采用放射免疫测定法检测脑cAMP、cGMP含量。结果:第二次缺氧时脑内兴奋性递质氨基酸含量明显降低,而脑内抑制性递质氨基酸、cAMP和cGMP含量明显升高,脑内氨基丁酸含量增高,可以延长耗氧时间。结论:急性缺氧适应能提高机体耐受缺氧能力,其机制可能与脑内兴奋性氨基酸水平降低,抑制性氨基酸水平升高有关。
Objective: To investigate the significance of changes of brain amino acid and second messenger in acute repetitive hypoxia. Methods: 72 mice were randomly divided into 4 groups. Group Ⅰ was saline control group, group Ⅱ was saline adaptation group, group Ⅲ was administration adaptation group, group Ⅳ was administration control group. Group Ⅲ and group Ⅳ were divided into three subgroups. Each group was given intraperitoneal injection of 25 mg / kg of L-AA, 60 mg / kg of sodium hydroxybutyrate and 20 μg / kg of 3-mercaptopropionic acid. The content of brain amino acids in each group was detected by RP-HPLC, and the contents of cAMP and cGMP in brain were detected by radioimmunoassay. Results: During the second hypoxia, the amino acid content of excitatory neurotransmitters in the brain decreased significantly. However, the levels of amino acids, cAMP and cGMP in brain were significantly increased, and the content of aminobutyric acid in the brain was increased, which could prolong the oxygen consumption time. Conclusion: Acute hypoxia adaptation can improve the tolerance of hypoxia to the body. The mechanism may be related to the decrease of excitatory amino acids and the increase of inhibitory amino acids.