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实验分为白介素－１（ＩＬ－１）预处理组和非预处理组，脂质体介导反义ＩＬ－１ 受体相关激酶－１（ＩＲＡＫ－１）寡核苷酸（ＯＤＮ） 转染ＨｅｐＧ２ 细胞，用ｗ ｅｓｔｅｒｎ 杂交分析ＩＲＡＫ－１ 表达水平，以夹心酶联免疫吸附测定法检测ＮＦ－κＢ含量． 结果表明，ＩＬ－１ 非预处理组反义ＩＲＡＫ－１ ＯＤＮ不能抑制ＩＲＡＫ－１ 表达和ＮＦ－κＢ活化，而预处理组ＩＲＡＫ－１ 表达和ＮＦ－κＢ活化受到明显抑制． 反义ＩＲＡＫ－１ ＯＤＮ 对ＮＦ－κＢ活化的抑制作用具有时间（５～２４ ｈ）和剂量（１～８ μｇ）依赖性． 说明ＩＬ－１ 预刺激在反义ＩＲＡＫ－１ ＯＤＮ抑制ＩＬ－１ 诱导的ＮＦ－κＢ活化中起决定性作用．
The experiment was divided into two groups: pretreatment with interleukin-1 (IL-1) and non-pretreatment with liposome-mediated transfection of antisense IL-1 receptor associated kinase-1 (ODN) HepG2 cells. The expression of IRAK-1 was analyzed by western hybridization and the content of NF-κB was detected by sandwich enzyme-linked immunosorbent assay (ELISA). The results showed that antisense IRAK-1 ODN could not inhibit IRAK-1 expression and NF-κB activation in IL-1 non-pretreatment group, while IRAK-1 expression and NF-κB activation in pretreatment group were significantly inhibited. The inhibitory effect of antisense IRAK-1 ODN on NF-κB activation was dependent on time (5-24 h) and dose (1-8 μg). Indicating that IL-1 pre-stimulation plays a decisive role in antisense IRAK-1 ODN inhibition of IL-1-induced NF-κB activation.