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目的 探讨肾移植长期存活受者不同免疫状态下外周血CC家族趋化因子及其受体的表达特征及临床意义。 方法 将 73例存活 3年以上的肾移植受者分为肾功能正常的对照组 (C组 )、低剂量免疫抑制组 (L组 )和慢性肾功能减退组 (D组 )。应用定量固相夹心酶联免疫吸附技术检测受者血浆中RANTES、单核细胞趋化蛋白 1(MCP 1)和巨噬细胞炎症蛋白 1α(MIP 1α)的含量 ,应用流式细胞技术检测外周血CCR5的表达率。 结果 L组的RANTES和MIP 1α含量及CCR5的表达率均明显低于C组 (P <0 .0 5 ) ,但三项指标在D、C组间差别无显著性意义 (P >0 .0 5 )。D组MCP 1含量显著高于C组 (P <0 .0 5 ) ,但在C、L组间差别无显著性意义 (P >0 .0 5 )。 结论 CC家族趋化因子及其受体表达与肾移植受者的免疫状态密切相关 ,可为临床评价和监测受者的免疫状态提供帮助
Objective To investigate the expression and clinical significance of CC family chemokines and their receptors in peripheral blood of different immune recipients with long-term renal transplant recipients. Methods 73 cases of renal transplant recipients who survived for more than 3 years were divided into control group (C group), low dose immunosuppressive group (L group) and chronic renal insufficiency group (D group). The levels of RANTES, monocyte chemoattractant protein 1 (MCP 1) and macrophage inflammatory protein 1α (MIP 1α) in plasma were detected by quantitative sandwich enzyme-linked immunosorbent assay (ELISA), and peripheral blood was detected by flow cytometry CCR5 expression rate. Results The expressions of RANTES, MIP 1α and the expression of CCR5 in group L were significantly lower than those in group C (P <0.05), but there was no significant difference between groups D and C (P> 0. 0) 5). The MCP 1 level in group D was significantly higher than that in group C (P <0.05), but there was no significant difference between group C and L (P> 0.05). Conclusion The expressions of chemokines and their receptors in CC family are closely related to the immune status of recipients of renal transplantation and may be helpful in clinical evaluation and monitoring of the immune status of recipients