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一、自动洗板机的应用及原理酶联免疫吸附测定(简称ELISA)主要是基于抗原或抗体能吸附至固相载体的表面并保持其免疫活性(称为包被),抗原或抗体与酶形成的酶结合物仍保持其免疫活性和酶催化活性的基本原理。在测定时,把受检标本(测定其中的抗体或抗原)和酶标抗原或抗体按不同的步骤与固相载体表面的抗原或抗体起反应,通过洗涤的方法使固相载体上形成的抗原抗体复合物与其他物质分开,最后结合在固相载体上的酶
First, the application and principle of automatic plate washer ELISA (ELISA) is mainly based on antigen or antibody can be adsorbed to the surface of the solid phase carrier and maintain its immunocompetence (called coating), antigen or antibody and enzyme The enzyme conjugate formed still retains the rationale for its immunocompetence and enzymatic activity. In the measurement, the test specimen (the antibody or the antigen to be measured therein) and the enzyme-labeled antigen or antibody are reacted with the antigen or antibody on the surface of the solid phase carrier in different steps and the antigen formed on the solid phase carrier The antibody complex separates from other substances and finally binds to the enzyme on a solid support