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革兰氏阴性杆菌感染时,肿瘤坏死因子(TNF)及白细胞介素-1(IL-1)是产生内毒素休克的主要原因。凝固酶阴性葡萄球菌是感染性疾病的重要病原,其细胞壁的某些组分与其所致的败血性休克密切相关。本文首先证实从临床分离的表皮葡萄球菌、金黄色葡萄球菌在体外能诱导人单核细胞分泌TNF,随后发现以上述两种细菌中分离的肽聚糖(PG)也有类似的诱导作用。PG对TNF的诱导效应在16h达到高峰,2h对TNF的分泌即达最高值的50%～60%,而作为对照的LPS(以大肠杆菌中提取)的诱导作用在2h时只达到最高值的4%。LPS对TNF的诱导能被多粘菌素所抑制,但PG的诱导效应不受多粘菌素的影响。
When Gram-negative bacilli are infected, tumor necrosis factor (TNF) and interleukin-1 (IL-1) are the main causes of endotoxic shock. Coagulase-negative staphylococci are important pathogens of infectious diseases, and some components of the cell wall are closely related to the septic shock they cause. This article first demonstrates that Staphylococcus epidermidis, isolated from clinical isolates of Staphylococcus aureus, induces the secretion of TNF by human monocytes in vitro and subsequently found similar induction of peptidoglycan (PG) isolated from both bacteria. The inducing effect of PG on TNF peaked at 16h, and reached the highest 50% ~ 60% at 2h, while the induction of LPS (extracted in E.coli) as the control only reached the highest value at 2h 4%. The induction of TNF by LPS can be inhibited by polymyxin, but the induction effect of PG is not affected by polymyxin.