论文部分内容阅读
目的探讨注射用免疫核糖核酸Ⅱ(BP素)对顺铂(DDP)的增效减毒作用及对S180荷瘤小鼠的免疫调节作用。方法建立小鼠S180皮下移植瘤模型,随机分组,采用腹腔注射给药,连续给药10 d,通过白细胞(WBC)计数、骨髓有核细胞计数,取肿瘤、胸腺、脾脏称重,计算抑瘤率、胸腺指数、脾脏指数以及MTT法检测刀豆蛋白A(Con A)诱导的小鼠脾脏T淋巴细胞增殖,观察BP素对顺铂的增效减毒作用。通过ELISA法检测血清中的IL-1β、IL-6、TNF-α水平,通过腹腔巨噬细胞对鸡红细胞吞噬率的测定评价BP素对S180荷瘤小鼠的免疫调节作用。结果 BP素与DDP联合应用可显著提高DDP对S180荷瘤小鼠的抗肿瘤作用(P<0.01),明显改善DDP化疗所致的脾脏指数下降,外周血WBC数、骨髓有核细胞数的减少及脾脏T淋巴细胞增殖抑制等毒副作用(P<0.01或P<0.05);单用BP素可显著降低荷瘤小鼠血清中的IL-1β、IL-6、TNF-α水平(P<0.01或P<0.05),提高腹腔巨噬细胞吞噬鸡红细胞的吞噬率(P<0.01)。结论 BP素对DDP具有增效减毒作用,并对S180荷瘤小鼠具有免疫调节作用。
Objective To investigate the synergic effect and attenuation of cisplatin (DDP) by immunosuppressive ribonucleic acid (BP) injection and its immunomodulatory effect on S180 tumor-bearing mice. Methods The subcutaneous xenograft models of S180 mice were established and randomly divided into groups. The mice were given intraperitoneal injection for 10 days. The numbers of bone marrow nucleated cells were counted by counting white blood cells (WBC), and the tumor, thymus and spleen were weighed, The thymus index, spleen index and MTT assay were used to detect the spleen T lymphocyte proliferation induced by Con A in mice. The synergistic and attenuated effects of BP on cisplatin were observed. Serum levels of IL-1β, IL-6 and TNF-α were detected by ELISA, and the immunoregulatory effect of BP on S180 tumor-bearing mice was evaluated by measuring peritoneal macrophage phagocytosis rate of chicken erythrocytes. Results The combination of BP and DDP could significantly increase the antitumor effect of DDP on S180 tumor-bearing mice (P <0.01), and significantly reduce the decrease of spleen index, the number of peripheral blood WBC and the number of bone marrow nucleated cells induced by DDP (P <0.01 or P <0.05). The level of IL-1β, IL-6 and TNF-α in sera of tumor-bearing mice was significantly lower than that of BP alone Or P <0.05), and increased phagocytosis rate of peritoneal macrophage phagocytosis chicken erythrocytes (P <0.01). CONCLUSION: BP has synergistic and attenuating effect on DDP and has immunomodulatory effect on S180 tumor-bearing mice.