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目的 :观察腹腔感染对兔多个器官系统的功能和形态结构的影响。 方法 :将 2 0只成熟期健康新西兰白兔随机分成感染组和对照组。感染组动物行盲肠结扎加穿孔 (CL P) ,对照组行盲肠探查术。两组动物均作颈内静脉置管术 ,输液维持 ,观察 1周。动态观察直肠温度、动脉血气、血清生化和尿中 3-甲基组氨酸排出量 ,记录动物死亡情况。在动物死亡或处死时抽血作细菌培养 ,取心、肺、肝、肾、小肠和脾等主要器官作光镜病理检查。 结果 :感染组动物在 CL P后直肠温度升高 ,血气分析异常 (Pa O2 下降 ,Pa CO2 升高和 p H值下降 ) ,血清生化异常 ,尿中 3-甲基组氨酸排出增多 ,血中细菌培养阳性率为 80 % ,器官病理改变显著 ,1周内动物均死亡 ,平均生存时间为 (84.1±39.0 ) h,与对照组比较有显著差异。 结论 :通过盲肠结扎加穿孔方法造成腹腔感染后 ,动物出现明显的器官功能和形态变化 ,具有多器官功能障碍综合征的特征。
Objective: To observe the effects of intraperitoneal infection on the function and morphological structure of multiple organ systems in rabbits. Methods: Twenty healthy mature New Zealand white rabbits were randomly divided into infection group and control group. Animals in the infection group were subjected to cecal ligation plus perforation (CLP) and control group cecal exploration. Two groups of animals were given for internal jugular vein catheterization, infusion maintenance, observed for 1 week. Dynamic observation of rectal temperature, arterial blood gas, serum biochemistry and urinary 3-methyl histidine output, record animal deaths. Blood was taken for bacterial culture at the time of death or sacrifice, and the major organs such as heart, lung, liver, kidney, small intestine and spleen were examined by light microscopy. Results: In the infected group, the rectal temperature increased, blood gas analysis was abnormal (Pa O2 decreased, Pa CO2 increased and p H decreased), serum biochemical abnormalities, urine 3-methyl histidine excretion increased, blood The positive rate of bacterial culture was 80%. The pathological changes of organs were significant. All the animals died within 1 week. The average survival time was (84.1 ± 39.0) h, which was significantly different from the control group. Conclusion: After abdominal cavity infection caused by cecal ligation and perforation, the animals showed obvious organ function and morphological changes, with the characteristics of multiple organ dysfunction syndrome.