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目的：用薄层色谱扫描法检测抗真菌药物氟康唑对真菌麦角固醇生物合成的影响。方法：真菌与不同浓度氟康唑温育２４ｈ后计数、称量、皂化，提取真菌中难皂化脂，薄层层析，扫描测定其中的麦角固醇和羊毛固醇含量。结果：随着氟康唑浓度的增加，真菌中的麦角固醇含量逐渐减少，而羊毛固醇含量迅速增加。当抑制８５％的麦角固醇生物合成时，真菌生长繁殖的受抑率也在８５％左右。结论：薄层色谱扫描法能准确测定真菌中的麦角固醇和羊毛固醇含量，可用于考察抗真菌药物对真菌麦角固醇生物合成的影响，为新化合物的体外抗真菌活性筛选和作用机制研究提供了新方法
OBJECTIVE: To determine the effect of antifungal fluconazole on the fungal ergosterol biosynthesis by TLC scanning. Methods: Fungi were incubated with different concentrations of fluconazole for 24 hours, then counted, weighed, and saponified. The contents of ergosterol and lanosterol in the fungi were determined by thin layer chromatography. Results: As the concentration of fluconazole increased, the content of ergosterol in fungi decreased gradually while the content of wool sterols increased rapidly. When inhibition of 85% ergosterol biosynthesis, fungal growth inhibition rate of about 85%. Conclusion: TLC-scanning method can accurately determine the content of ergosterol and lanosterol in fungi, and can be used to study the antifungal effect on fungal ergosterol biosynthesis. Screening of antifungal activity of new fungi and its mechanism of action Provides a new way