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目的:分析叶酸代谢相关酶基因多态性与不良孕产的临床关系。方法:使用基因测序技术检测珠海市妇幼保健院不良孕产妇女200例(观察组)及健康妇女300例(对照组)的甲硫氨酸合酶还原酶(MTRR)A66G基因多态性以及亚甲基四氢叶酸还原酶(MTHFR)C677T、A1298基因多态性,使用Logistic回归模型分析叶酸代谢基因-基因交互作用。结果:观察组妇女MTHFR A1298基因型分布频率13.00%较对照组4.00%高;观察组妇女MTHFR C677T基因型分布频率9.67%较对照组2.50%高,差异具有统计学意义(P<0.05);MTRR A66G突变位点和MTHFR A1298突变位点存在交互作用,(OR=1.51,P=0.021)。结论:MTHFR A1298和C677T与不良孕产的发生有一定关系。
Objective: To analyze the clinical relationship between folic acid metabolism-related enzyme gene polymorphism and adverse pregnancy. Methods: DNA sequencing was used to detect methionine synthase reductase (MTRR) A66G polymorphism in 200 pregnant women and 200 healthy women in control group. Methyltetrahydrofolate reductase (MTHFR) C677T, A1298 gene polymorphism, the use of Logistic regression model analysis of folic acid metabolism gene - gene interaction. Results: The distribution frequency of MTHFR A1298 genotype in observation group was 13.00% higher than that in control group. The distribution frequency of MTHFR C677T genotype in observation group was 9.67% higher than that in control group (2.50%), the difference was statistically significant (P <0.05); MTRR There was interaction between A66G mutation site and MTHFR A1298 mutation site (OR = 1.51, P = 0.021). Conclusion: MTHFR A1298 and C677T have some relationship with the occurrence of adverse pregnancy.