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在3-氨丙基三乙氧基硅烷(APTES)功能化修饰后的Fe3O4纳米粒子溶液体系中,加入乙酸银使游离态银离子充分吸附在磁粒的表面,与氨基化基团形成配位化合物,再利用甲酸钠将配位银离子还原为银,制成磁性Fe3O4/Ag复合纳米粒子。采用紫外吸收可见光谱(UV-Vis)、电子能谱仪(EDS)、透射电子显微镜(TEM)和超导量子干涉仪(SQUID)等方法对复合粒子的形态、结构、组成以及磁学性质进行表征。同时,采用大肠杆菌和金黄色葡萄球菌对磁性Fe3O4/Ag复合纳米粒子的抗菌性能进行研究。结果表明:合成的复合粒子平均粒径为30 nm,室温下磁化强度为2.0(A·m2)/kg;磁性Fe3O4/Ag复合纳米粒子对大肠杆菌和金黄色葡萄球菌具有显著地抑制效果,指定实验条件下在24 h后两种细菌的存活量在0.11×103(CFU)以下,进一步提高磁性Fe3O4/Ag复合纳米粒子用量,大肠杆菌存活量几乎100%减灭。本研究方法为制备具有外场响应功能(如磁场响应)的新型纳米生物抗菌材料提供了很好的范例。
In the system of 3-aminopropyltriethoxysilane (APTES) functionalized Fe3O4 nanoparticle solution, silver acetate is added to make the free silver ion fully adsorb on the surface of the magnetic particle to form a coordination compound with the amino group , And then use sodium formate to reduce the coordination silver ions into silver to make magnetic Fe3O4 / Ag composite nano-particles. The morphology, structure, composition and magnetic properties of the composite particles were characterized by UV-Vis, EDS, TEM and SQUID. Characterization. At the same time, the antibacterial properties of magnetic Fe3O4 / Ag composite nanoparticles were studied by using Escherichia coli and Staphylococcus aureus. The results showed that the average particle size of synthesized composite particles was 30 nm and the magnetization was 2.0 (A · m2) / kg at room temperature. Magnetic Fe3O4 / Ag composite nanoparticles had a significant inhibitory effect on Escherichia coli and Staphylococcus aureus. Under the experimental conditions, the viability of the two bacteria was below 0.11 × 103 (CFU) after 24 h, and the amount of magnetic Fe3O4 / Ag composite nanoparticles was further increased, and the viability of E. coli was reduced by almost 100%. The present method provides a good example for the preparation of a novel nanobiological antimicrobial material with field response function such as magnetic field response.