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将致病性温和气单胞菌 (99- 5- A)制成菌苗 ,按 0 .2 5ml/尾的剂量给健康无病的“尼罗”、“奥利亚”、“尼奥”三种罗非鱼作腹腔注射。结果为 :(1)免疫后第 10天 ,三种罗非鱼血清的抗体效价分别为 1∶ 8、1∶ 16和 1∶ 16。(2 )抗体含量的高峰期分别出现在第 2 2天 ,1∶ 6 4维持 6天 ;第 2 2天 ,1∶ 32维持 3天 ;第 16天 ,1∶ 6 4维持 9天。 (3) 3个月后免疫鱼血清抗体效价分别为 1∶ 8、1∶ 4和 1∶ 16。而对照组 3个月内均没有检测到抗体。 (4 ) 3个月后免疫保持率分别为10 0 %、75%和 10 0 %
The pathogenic strains of Aeromonas sobria (99- 5 A) were made into bacterins, which were given to healthy healthy “Nile”, “Oriya”, “Neo” at a dose of 0.25 ml / Three kinds of tilapia for intraperitoneal injection. The results were as follows: (1) On the 10th day after immunization, the titers of the three tilapia sera were 1:8, 1:16 and 1:16, respectively. (2) The peak of antibody content appeared on day 22, 1: 6 4 for 6 days, on day 2 2, 1:32 for 3 days, on day 16, 1:46 for 9 days. (3) The serum antibody titers of immunized fish after 3 months were 1:8, 1:4 and 1:16, respectively. The control group did not detect antibodies within 3 months. (4) After 3 months, the immune maintenance rates were 100%, 75% and 100%