牛血样品通过低温冷冻、解冻及离心处理,除去细胞碎片后分成2份,1份为低水平,另1份加入Se~(+4)(高水平)。2份样品分别以2ml/瓶的量分装在棕色瓶中,冷冻干燥,辐射灭菌,最后于0℃以下保存。用荧光分光光度法测其均匀性与稳定性。结果本标准物质至少可以稳定1年。采用多个实验室多种方法确定的标准值为:低水平:(99.9±6.9)μg/L,高水平:(981±42)μg/L。 Bovine blood samples were cryogenically frozen, thawed and centrifuged to remove cell debris and divided into two fractions, one low and one additional Se (+4) (high). Two samples were separately dispensed in 2ml / bottle in brown bottles, lyophilized, sterilized by irradiation and finally stored at 0 ° C. Fluorescence spectrophotometry was used to measure the uniformity and stability. Results The standard material can be stable for at least 1 year. The standard values ​​determined using multiple methods in multiple laboratories were: low (99.9 ± 6.9) μg / L and high (981 ± 42) μg / L.