端粒不稳定与肿瘤发生有关,永生化细胞中端粒虽然缩短,但由于端粒酶激活使端粒保持稳定。近年来有报道端粒酶中催化功能亚基对端粒酶活性的作用较RNA部分更加重要,端粒酶激活是细胞永生化与肿瘤发生的关键。在正常卵巢上皮细胞中端粒酶阴性,而在卵巢癌细胞及腹水肿瘤细胞中端粒酶呈阳性。本研究以SV-40大T抗原转染有乳腺癌/卵巢癌家族史妇女的卵巢上皮细胞系(FHIOSE)及无此家族史妇女的卵巢上皮细胞系(NFHIOSE),比较两种转染细胞的生长能力、平均端粒长度及端 Telomere instability is associated with tumorigenesis. Although telomeres are shortened in immortalized cells, telomeres remain stable due to telomerase activation. In recent years, it has been reported that the catalytic functional subunit of telomerase is more important to the telomerase activity than the RNA part, and telomerase activation is the key to cell immortalization and tumorigenesis. Telomerase was negative in normal ovarian epithelial cells, whereas telomerase was positive in ovarian cancer cells and ascites tumor cells. In this study, the ovarian epithelial cell line (FHIOSE) with a family history of breast cancer/ovarian cancer was transfected with SV-40 large T antigen and the ovarian epithelial cell line (NFHIOSE) without this family history was compared between the two transfected cells. Growth ability, average telomere length and end