论文部分内容阅读
离子色谱具有方便、快速、灵敏度高、选择性好、重现性好、准确度高、可同时分析多种离子化合物等优点,其中的高效阴离子交换色谱-脉冲安培检测法(HPAEC-PAD)可直接分析糖类物质,可应用于多糖疫苗及多糖蛋白结合疫苗中多糖的分析。近年来,离子色谱应用在多糖及多糖蛋白结合疫苗的质量控制中,如测定疫苗中的多糖、游离糖、杂质多糖、对人有免疫原性的功能基团等的研究有许多报道,对有关内容进行了综述。
Ion chromatography has the advantages of convenient, fast, high sensitivity, good selectivity, good reproducibility, high accuracy, simultaneous analysis of a variety of ionic compounds, among which the high performance anion exchange chromatography-pulse amperometric detection (HPAEC-PAD) Direct analysis of carbohydrates can be applied polysaccharide vaccine and polysaccharide protein conjugate vaccine polysaccharide analysis. In recent years, the application of ion chromatography in polysaccharide and polysaccharide protein conjugate vaccine quality control, such as the determination of vaccine polysaccharides, free sugars, impurity polysaccharides, immunogenicity of human functional groups have many reports on the relevant The content is reviewed.