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为了探索Ｇ蛋白及ｃＡＭＰ -ＰＫＡ信号系统在缺氧条件下梭曼毒性升高机制中的作用 ,作者用Ｗｅｓｔｅｒｎｂｌｏｔ检测Ｇｉａ、Ｇｑａ含量、放射免疫测定ｃＡＭＰ浓度及酶放射化学分析测定ＰＫＡ活性。结果显示 :梭曼中毒、单一缺氧损伤和缺氧复合梭曼中毒大鼠脑组织Ｇｐａ含
In order to explore the role of G-protein and cAMP-PKA signaling in the mechanism of soman toxicity in hypoxia, we examined the Gia and Gqa levels by Western blot, cAMP concentration by radioimmunoassay and PKA activity by enzyme-linked immunosorbent assay. The results showed that soman intoxication, single anoxia injury and anoxia combined with soman intoxication rat brain tissue Gpa