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目的 研究肺支气管良恶性病变中DNA倍体及DNA指数(DI)情况;探讨DNA含量在癌变过程中的变化特点。方法用图像分析系统(MPIAS—500)对39例支气管粘膜慢性炎、45例支气管粘膜上皮鳞状上皮化生、45例支气管粘膜上皮异型增生、63例支气管肺癌进行DNA倍体分布及DNA指数的检测。结果 支气管粘膜慢性炎组、鳞状上皮化生组非整倍体均<1.3％,DI均值分别为0.92和1.03;异型增生组非整倍林为2.8％,DI均值为1.34;而肺癌组非整体为18.93％,DI均值为1.72,明显高于良性病变组(P<0.001)。结论 肺支气管良、恶性病变细胞核DNA倍体和非整倍体中所占的百分比不同;支气管粘膜上皮细胞在癌变过程中核DNA含量逐渐增多,核型分布范围增宽,非整倍体细胞增多,DI均值呈递增趋势。
Objective To study DNA ploidy and DNA index (DI) in benign and malignant bronchial lesions, and to explore the changes of DNA content during carcinogenesis. Methods DNA ploidy distribution and DNA index were performed in 39 cases of chronic bronchial mucosal inflammation, 45 cases of bronchial epithelial squamous metaplasia, 45 cases of bronchial epithelial dysplasia, and 63 cases of bronchial lung cancer using image analysis system (MPIAS-500). Testing. Results The aneuploidy of bronchial mucosal chronic inflammation group and squamous metaplasia group were all less than 1.3%, and the DI values were 0.92 and 1.03 respectively. The atypical hyperplasia group was 2.8% and the mean DI value was 1.34; while the lung cancer group was not. The overall rate was 18.93% and the mean DI value was 1.72, which was significantly higher than that in the benign lesion group (P<0.001). Conclusion The percentage of nuclear DNA ploidy and aneuploidy in the lung bronchial benign and malignant lesions are different. The nuclear DNA content of the bronchial epithelial cells gradually increases during the process of carcinogenesis, the karyotype distribution range widens, and the aneuploid cells increase. The mean value of DI is increasing.