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目的:建立同时测定桂枝、白芍药对提取物中8个成分(没食子酸、氧化芍药苷、芍药内酯苷、芍药苷、1,2,3,4,6-五没食子酰葡萄糖、香豆素、肉桂酸、苯甲酰芍药苷)含量的高效液相色谱法。方法:采用Agilent Eclipse XDB-C18色谱柱(250mm×4.6 mm,5μm),以乙腈(A)-0.1%磷酸水溶液(B)为流动相,梯度洗脱,流速为1.0 mL·min-1,检测波长为267 nm(0～13 min,检测没食子酸)、258 nm(13～20 min,检测氧化芍药苷)、230 nm(20～35 min,检测芍药内酯苷、芍药苷)、274 nm(35～47 min,检测1,2,3,4,6-五没食子酰葡萄糖)、280 nm(47～61 min,检测香豆素、肉桂酸)、230 nm(61～70 min,检测苯甲酰芍药苷),柱温为30℃。结果:桂枝、白芍药对提取物中8个成分没食子酸、氧化芍药苷、芍药内酯苷、芍药苷、1,2,3,4,6-五没食子酰葡萄糖、香豆素、肉桂酸、苯甲酰芍药苷进样量分别在0.0936～0.936μg(r=0.9995)、0.005320～0.05320μg(r=0.9991)、0.1365～1.365μg(r=0.9996)、0.1456～1.456μg(r=0.9996)、0.0939～0.939μg(r=0.9997)、0.02678～0.2678μg(r=0.9992)、0.1336～1.336μg(r=0.9993),0.01938～0.1938μg(r=0.9992)与峰面积呈良好的线性关系;平均回收率(n=5)分别为99.8%、99.2%、99.0%、99.1%、98.3%、100.0%、98.1%、99.9%。结论:该方法准确可靠、重复性好,可用于桂枝、白芍药对提取物的质量控制。
OBJECTIVE: To establish a method for simultaneous determination of eight components (gallic acid, paeoniflorin, paeoniflorin, paeoniflorin, 1,2,3,4,6-decalin, coumarin Suan, cinnamic acid, benzoyl paeoniflorin) content of high performance liquid chromatography. Methods: The mobile phase was eluted with an Agilent Eclipse XDB-C18 column (250 mm × 4.6 mm, 5 μm) using a mobile phase of acetonitrile (A) -0.1% phosphoric acid (B) at a flow rate of 1.0 mL · min -1 The detection wavelength was 267 nm (0 to 13 min, the detection of gallic acid), 258 nm (13 to 20 min, detection of paeoniflorin), 230 nm (20 to 35 min, detection of paeoniflorin and paeoniflorin), 274 nm 35-47 min, detection of 1,2,3,4,6-decalloylglucose), 280 nm (47-61 min, detection of coumarin, cinnamic acid), 230 nm Acyl paeoniflorin), the column temperature is 30 ℃. Results: Guizhi and Paeonia lactiflora were sensitive to 8 components of gallic acid, paeoniflorin, paeoniflorin, paeoniflorin, 1,2,3,4,6-decalloylglucose, coumarin and cinnamic acid (R = 0.9991), 0.1365 ~ 1.365μg (r = 0.9996), 0.1456 ~ 1.456μg (r = 0.9996) , 0.0939 ~ 0.939μg (r = 0.9997), 0.02678 ~ 0.2678μg (r = 0.9992), 0.1336 ~ 1.336μg (r = 0.9993) and 0.01938 ~ 0.1938μg (r = 0.9992) The recoveries (n = 5) were 99.8%, 99.2%, 99.0%, 99.1%, 98.3%, 100.0%, 98.1%, 99.9%, respectively. Conclusion: The method is accurate, reliable and reproducible. It can be used for the quality control of extracts of Guizhi and Paeonia.