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目的探讨核因子-κB(NF-κB)、血管细胞间黏附分子-1(VCAM-1)在人冠状动脉粥样硬化斑块中的表达及其与斑块稳定性之间的关系。方法从48例尸检标本中获得48个冠状动脉前降支标本。通过苏木素-伊红染色病理特征将标本分为正常对照组、稳定斑块组和不稳定斑块组。采用免疫组织化学法检测NF-κB、VCAM-1在各组冠状动脉内膜中的表达。结果正常对照组NF-κB、VCAM-1不表达,在稳定斑块组和不稳定斑块组NF-κB、VCAM-1表达增加(P<0.05),且不稳定斑块组表达高于稳定斑块组(P<0.05)。NF-κB、VCAM-1在粥样硬化冠状动脉内膜区域的表达强度呈正相关(r=0.401,P<0.05)。结论NF-κB、VCAM-1参与冠状动脉粥样硬化斑块的发生、发展并与斑块稳定性密切相关。NF-κB作为引发炎症的关键转录因子可能上调VCAM-1的表达。
Objective To investigate the expression of nuclear factor-κB (NF-κB) and vascular cell adhesion molecule-1 (VCAM-1) in human coronary atherosclerotic plaque and its relationship with plaque stability. Methods 48 specimens of anterior descending coronary artery were obtained from 48 autopsy specimens. The specimens were divided into normal control group, stable plaque group and unstable plaque group by hematoxylin-eosin staining pathological features. Immunohistochemistry was used to detect the expression of NF-κB and VCAM-1 in the intima of coronary arteries. Results The expressions of NF-κB and VCAM-1 in stable plaque group and unstable plaque group were increased (P <0.05), and the expression of VCAM-1 in unstained plaque group was higher than that in normal plaque group Plaque group (P <0.05). The expression of NF-κB and VCAM-1 in the intima of coronary atherosclerosis was positively correlated (r = 0.401, P <0.05). Conclusion NF-κB and VCAM-1 are involved in the development of coronary atherosclerotic plaque and are closely related to the plaque stability. NF-κB, a key transcription factor that triggers inflammation, may up-regulate the expression of VCAM-1.