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合成了一种结构易控、化学稳定性好、呈电中性及电化学活性高的含磷三足配体N-二(4-乙基苯-二氨基甲酰基)甲基磷酸及其稀土Eu(Ⅲ)配合物,采用了~1HNMR、~(13)CNMR、红外光谱、元素分析、差热-热重及连续物质的量比法对其组成进行表征并推断其结构,确定了配体与稀土离子形成了1∶1型配合物Eu(pic)3L。在进行配合物与DNA相互作用实验中,考察了扫描速率ν、p H、配合物浓度和离子强度因素对其的影响。实验结果表明,Eu(pic)3L与DNA碱基进行相互结合从而形成非电活性物质,使游离的Eu(pic)3L浓度降低,Eu(pic)3L的峰电流减小,说明Eu(pic)3L与DNA的相互作用是以嵌插形式结合的。根据Eu(pic)3L的电化学活性研究了以该配合物为杂交探针在DNA电化学传感器方面的应用。结果发现,Eu(pic)3L在修饰单链DNA的电极检测作用时,并无明显的电化学信号响应;检测杂交双链DNA时,却出现了明显信号,与此同时Eu(pic)3L的DNA电化学传感器对DNA的互补、碱基错配及非互补序列均有良好的识别功能。
A three-legged ligand N-di (4-ethylbenzene-dicarbamoyl) methylphosphonic acid with high chemical stability and good electroneutrality and electrochemical activity was synthesized and its rare earth Eu (Ⅲ) complexes were synthesized and characterized by ~ 1HNMR, ~ (13) CNMR, IR, elemental analysis, DTA-TG method and quantum ratio method With rare earth ions to form a 1: 1 complex Eu (pic) 3L. In the experiment of the interaction between the complex and DNA, the influence of scan rate ν, p H, complex concentration and ionic strength was investigated. The experimental results show that the Eu (pic) 3L and Eu (pic) 3L are combined with each other to form a non-electroactive substance. The concentration of free Eu (pic) 3L decreases and the peak current of Eu The interaction of 3L with DNA is by intercalation. Based on the electrochemical activity of Eu (pic) 3L, the use of this complex as a hybridization probe in DNA electrochemical sensors has been investigated. The results showed that Eu (pic) 3L had no obvious electrochemical signal response when detecting the single-stranded DNA. When the double-stranded DNA was hybridized, Eu (pic) 3L showed a clear signal. At the same time, Eu (pic) DNA electrochemical sensors for DNA complementarity, base mismatch and non-complementary sequences have good recognition.