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以酶、抗体相结合作为生物传感器的识别元件进行抗原的检测，不仅在临床医学上，而且在生化战剂的侦检中都有着广阔的应用前景。本文所研究的是将镀铂玻碳电极以化学吸附和交联的方式固定化葡萄糖氧化酶（ＧＯＤ），然后进一步耦联葡萄球菌肠毒素Ｃ１型抗体（Ａｎｔｉ－ＳＥＣ１），从而构成一种新型的电流型ＳＥＣ１酶免疫传感器。当抗原（ＳＥＣ１）与抗体发生免疫反应而形成抗原－抗体络合物后，使与抗体耦联的酶的活性受到调制，电极的响应信号发生变化，从而达到检测抗原的目的。实验表明，以化学吸附和交联两种方式制得的酶免疫电极在检测抗原时，与响应电流存在很好线性关系的浓度范围分别为２０～１００μｇ／Ｌ、３～１８μｇ／Ｌ。
The detection of antigen by the combination of enzyme and antibody as the identification element of the biosensor has a broad application prospect not only in clinical medicine but also in the detection of biological and chemical warfare agents. The purpose of this paper is to immobilize glucose oxidase (GOD) by chemisorption and cross-linking of platinum-plated glassy carbon electrode and then to further couple anti-SEC1 with anti-SEC1 to form a novel Current-based SEC1 enzyme immuno-sensor. After the antigen (SEC1) immunologically reacts with the antibody to form an antigen-antibody complex, the activity of the enzyme coupled to the antibody is modulated and the response signal of the electrode is changed to achieve the purpose of antigen detection. The experimental results showed that there was a good linear relationship between the response time and the electrochemical response of the enzyme-immunized electrode prepared by chemisorption and cross-linking in the range of 20 ~ 100μg / L and 3 ~ 18μg / L, respectively.