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目的:研究基质金属蛋白酶-2(MMP-2)si RNA对口腔鳞状细胞癌Tca8113细胞凋亡的作用。方法:采用MMP-2 si RNA慢病毒感染Tca8113,q PCR检测MMP-2表达,MTT检测细胞活性,Annexin-V单染检测细胞凋亡。结果:MMP-2 si RNA慢病毒成功抑制Tca8113中MMP-2 m RNA表达。MMP-2干扰组细胞活性显著低于阴性病毒组(P<0.01),且细胞凋亡率高于阴性对照组(P<0.01)。结论:MMP-2干扰能促进Tca8113凋亡,起到抗肿瘤效应。
AIM: To investigate the effect of matrix metalloproteinase-2 (si RNA) on apoptosis in oral squamous cell carcinoma Tca8113 cells. Methods: Tca8113 was transfected with MMP-2 si RNA lentivirus and the expression of MMP-2 was detected by q-PCR. The cell viability was detected by MTT and the apoptosis was detected by Annexin-V staining. Results: The MMP-2 si RNA lentivirus successfully inhibited the expression of MMP-2 mRNA in Tca8113. The cell activity of MMP-2 interference group was significantly lower than that of negative virus group (P <0.01), and the apoptosis rate was higher than that of negative control group (P <0.01). Conclusion: MMP-2 interference can promote the apoptosis of Tca8113 and play an anti-tumor effect.