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通过对几种培养基比较试验,筛选出WPM是早熟油桃胚培养的最适培养基,其最高萌发率为95.56％;不同熟期成熟的早熟油桃品种对低温处理的要求时间不同,愈是早熟的品种对低温要求时间愈长,晚熟品种相对较短;1mg／L 6-BA+0.2mg／LIBA+0.1 mg/L GA。能有效破除早熟油桃的休眠,而且幼苗生长良好。早熟油桃胚培养培养基的pH值要求范围为5.7-6.2,其中愈是早熟对培养基pH值的要求相对较高;对试管苗的移栽采用白天口杯罩住,夜间揭开,持续5-7 d,3 d多菌灵灌根1次,15 d后发现腐烂子叶及时清除,能有效提高试管苗的成活率。
Through comparing several kinds of culture medium, WPM was selected as the optimum culture medium for precocious nectarie embryo culture, and the highest germination rate was 95.56%. The different mature time required for low temperature treatment of mature early nectarine cultivars The premature varieties required the longer time for low temperature, the late-maturing varieties were relatively short; 1mg / L 6-BA + 0.2mg / LIBA + 0.1 mg / L GA. Can effectively get rid of early nectarine dormancy, and seedlings grow well. Premature peach embryo culture medium pH value range of 5.7-6.2, of which the more premature on the medium pH value is relatively high; the transplanting of tube seedlings covered with day cup, open at night, sustained 5-7 d, 3 d carbendazim once, after 15 d found rotting cotyledons removed in time, can effectively improve the survival rate of in vitro seedlings.