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目的:观察地耳草总黄酮对5/6肾切除慢性肾功能衰竭(CRF)大鼠的作用及可能机制。方法:采用5/6肾切除复制大鼠CRF模型。实验分为模型对照组、阳性对照1组(金水宝胶囊)、阳性对照2组(卡托普利片),地耳草提取物低、中、高剂量组,假手术组共7组。灌胃给予相应药物或0.5%CMC-Na溶液1ml/100g。分别于给药28天、57天和89天,各组随机抽取部分大鼠腹主动脉采血,血清尿素氮(Bun)、肌酐(Cr)、层粘连蛋白(laminin,LN);处死大鼠,取肾脏固定,对肾脏进行切片,HE、Masson染色完成病理组织学检查,定量分析肾组织纤维化程度,免疫组化法检测转化生长因子-β1(TGF-β1)在肾组织中的表达。结果:连续给药28、57、89天,地耳草提取物各剂量组对5/6肾切除慢性肾功能衰竭大鼠均有一定的治疗作用,能降低模型大鼠Cr、Bun水平;降低血清LN含量及肾组织TGF-β1表达,使残余肾脏的肾小球、肾小管、肾间质病变及纤维化程度明显减轻,以120mg/kg、180mg/kg剂量组作用明显。结论:地耳草能改善慢性肾功能衰竭大鼠肾功能,其作用可能与通过TGF-β1途径改善肾组织纤维化病变程度有关。
Objective: To observe the effect and possible mechanism of total flavonoids of Eriocheir sinensis on 5/6 nephrectomy in chronic renal failure (CRF) rats. Methods: Rat model of CRF was reproduced by 5/6 nephrectomy. The experiment was divided into model control group, positive control group 1 (Jinshuibao capsule), positive control group 2 (Captopril tablets), low, medium and high dose group of Elaera extract group and sham operation group. Gavage given the corresponding drug or 0.5% CMC-Na solution 1ml / 100g. Rats in each group were randomly selected from the abdominal aorta, serum urea nitrogen (Bun), creatinine (Cr) and laminin (LN) at 28 days, 57 days and 89 days after administration. The kidneys were fixed, the kidneys were sectioned, HE and Masson stained histopathological examination, quantitative analysis of renal tissue fibrosis, immunohistochemistry detection of transforming growth factor-β1 (TGF-β1) in renal tissue expression. Results: After continuous administration for 28, 57, and 89 days, each dose group of Eriocheir sinensis had a certain therapeutic effect on 5/6 nephrectomy rats with chronic renal failure, which could reduce Cr and Bun levels in model rats and decrease The level of serum LN and the expression of TGF-β1 in renal tissue significantly reduced the degree of glomerular, tubular and renal interstitial lesions and the degree of fibrosis in the residual kidney. The effects of 120mg / kg and 180mg / kg were significant. Conclusion: Eriocae can improve renal function in rats with chronic renal failure, which may be related to the improvement of the degree of renal fibrosis by TGF-β1 pathway.