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目的 评价结核分支杆菌MPT6 4、ESAT6DNA疫苗的保护效力和细胞因子的增强作用。方法 将BALB/C小鼠随机分为 14组 ,分别用ESAT6 (2 5 μg) +MPT6 4 (2 5 μg) (A组 ) ,ESAT6 (10 0 μg) +IFN γ(10 0 μg) (B组) ,ESAT6 (75 μg) +MPT6 4 (2 5 μg) (C组 ) ,ESAT6 (10 0 μg) +IL 12 (10 0 μg) (D组 ) ,MPT6 4 (10 0 μg) +IL 12 (10 0 μg) (E组 ) ,ESAT6 (2 5 μg) +MPT6 4 (75 μg) (F组 ) ,MPT6 4 (10 0 μg) (G组 ) ,PvaX1载体质粒 (10 0 μg) (H组) ,ESAT6 (10 0 μg) (I组 ) ,ESAT6 (10 0 μg) +MPT6 4 (10 0 μg) (J组 ) ,ESAT6 (5 0 μg) +MPT6 4 (5 0 μg) (K组) ,MPT6 4 (10 0 μg) +IFN γ(10 0 μg) (L组 ) ,卡介苗(M组) ,生理盐水 (N组 )免疫小鼠。每组各 6只小鼠免疫 8周后以结核分支杆菌H37Rv小鼠尾静脉攻击小鼠。攻击 4周后 ,观察肺、肝、脾组织的病理改变。结果 结核分支杆菌攻击后 ,肺组织病理改变为 :以渗出性反应为主的病变有N组 ,表现为肺泡壁毛细血管充血 ,肺泡腔内充满渗出液 ;以增殖性反应为主的病变为M、J组 ,表现为较多淋巴细胞、泡沫样细胞、上皮样细胞、郎罕细胞组成的结核肉芽肿 ,并可见肺泡壁组织增生增厚 ;其余均为增殖性与渗出性反应的混合性病变 (A、B、C、D、E、
Objective To evaluate the protective effect of Mycobacterium tuberculosis MPT6 4 and ESAT6 DNA vaccine and the enhancement of cytokines. Methods BALB / C mice were randomly divided into 14 groups: ESAT6 (25 μg) + MPT6 4 (25 μg) (group A), ESAT6 (100 μg) + IFN γ , ESAT6 (75 μg) + MPT6 4 (25 μg) (group C), ESAT6 (100 μg) + IL 12 (group D), MPT6 4 (10 μg) (E group), ESAT6 (25 μg) + MPT6 4 (75 μg) (group F), MPT6 4 (100 μg) ESAT6 (100 μg) (group I), ESAT6 (100 μg) + MPT6 4 (100 μg) (group J), ESAT6 (50 μg) + MPT6 4 ), MPT6 4 (10 0 μg) + IFN γ (10 0 μg) (group L), BCG (group M) and normal saline (group N). Mice were challenged with the tail vein of Mycobacterium tuberculosis H37Rv mice after 8 weeks of immunization of 6 mice in each group. After 4 weeks of attack, the pathological changes of lung, liver and spleen were observed. Results Mycobacterium tuberculosis attack, the pathological changes of lung tissue: exudative reaction-based lesions in group N, manifested as alveolar capillary congestion, alveolar cavity filled with exudate; proliferative reaction-based lesions M, J group, showed more lymphocytes, foam-like cells, epithelial cells, Langerhans cells consisting of tuberculosis granuloma, and visible alveolar wall hyperplasia thickening; the rest are proliferative and exudative reaction Mixed lesions (A, B, C, D, E,