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目的明确血清骨保护素(osteoprotegerin,OPG)在维吾尔族、汉族老年人是否存在差异性,探讨其与腰椎、左股骨近端骨密度T值(BMD-T)的关系。方法选择2011年10月至2013年12月在新疆医科大学第一附属医院住院患者312例(年龄≥60岁),采用双能X线吸收法(DXA)测量腰椎(L)、左股骨近端BMD-T值,采用酶联免疫吸附法(ELISA)检测血OPG的浓度,进行统计学分析。结果 (1)血OPG浓度在低骨量组1.588(1.559)较骨量正常组2.062(1.606)降低(Z=1.531,P=0.018)。(2)血OPG浓度在维吾尔族组1.503(1.033)较汉族组1.971(1.805)偏低(Z=1.834,P=0.002);按性别分层:维吾尔族男性血OPG浓度1.474(0.729)较汉族男性2.062(1.712)明显偏低(Z=2.215,P=0.000),而在女性间两民族血OPG浓度则无统计学差异;按病例-对照分层:维吾尔族骨量正常者血OPG浓度1.772(1.044)明显较汉族骨量正常者2.303(2.031)低(Z=1.404,P=0.039),而在低骨量者两民族血OPG浓度则无统计学差异。(3)Spearman相关性分析结果显示:汉族女性L2、L3、L4、L总(LZ)、股骨颈(Neck)、大粗隆(G.T)、粗隆间(Inter Tro)、髋关节(Hip)部位血OPG浓度与BMD-T值呈负相关(r=-0.333~-0.235,P=0.02~0.032),汉族男性G.T-T与血OPG浓度呈正相关(r=0.174,P=0.026),维吾尔族无论男性、女性血OPG浓度与各骨骼部位BMD-T值无明显相关性(P>0.05),经控制BMI、年龄后汉族女性血OPG浓度与L2、L3、L总、Inter Tro、Hip部位BMD-T值的相关性仍有统计学意义;(4)二元线性回归分析结果提示:汉族女性血OPG浓度对L1、L2、L3、L总、Inter Tro部位T值是负性决定因素,可以解释T值变异的0.8%~10%。(5)拟合曲线提示:汉族女性血OPG浓度与L1、L2、L3、L总、Inter Tro部位T值以线性拟合最优,与G.T、Hip部位T值以二次多项式拟合最优,与L4、Neck部位T值无最优拟合曲线。结论维吾尔族老年人血OPG浓度较汉族老年人偏低,尤其维吾尔族男性低于汉族男性;血OPG浓度变化可能是汉族老年女性骨密度T值变化的影响因素。
Objective To determine whether osteoprotegerin (OPG) is related to the elderly Uighur and Han nationality, and to explore its relationship with BMD-T in the lumbar and proximal femur. Methods From October 2011 to December 2013, 312 patients (aged ≥60 years) with inpatients at the First Affiliated Hospital of Xinjiang Medical University were enrolled in this study. Lumbar vertebral (L), proximal femur BMD-T value, using the enzyme-linked immunosorbent assay (ELISA) to detect the blood OPG concentration for statistical analysis. Results (1) The level of blood OPG decreased at 1.588 (1.559) in the low bone mass group and 2.062 (1.606) in the normal bone mass group (Z = 1.531, P = 0.018). (2) The level of OPG in blood of Uygur group was lower than that of Han nationality group at 1.503 (1.033) (1.933, 1.805) (Z = 1.834, P = 0.002) There was no significant difference in the blood OPG concentrations between the two ethnic groups in females. According to the case-control stratification, the blood OPG concentration in normal Uighur group was 1.772 (1.044) was significantly lower than that of 2.303 (2.031) normal Han (Z = 1.404, P = 0.039), while there was no significant difference in OPG concentration between two ethnic groups with low bone mass. (3) The results of Spearman correlation analysis showed that L2, L3, L4, L, Neck, GT, Inter Tro, Hip, There was a negative correlation between serum OPG concentration and BMD-T (r = -0.333-0.235, P = 0.02-0.032). There was a positive correlation between GT-T and blood OPG concentration in Han men (r = 0.174, P = 0.026) There was no significant correlation between serum OPG concentration and the BMD-T value of each skeletal group in both males and females (P> 0.05). After control of BMI and age, there was no significant correlation between the level of OPG in L2, L3, L, Inter Tro and Hip (4) The results of binary linear regression analysis suggested that the blood OPG concentration in Han women was a negative determinant of T values of L1, L2, L3, L total and Inter Tro sites, Can explain the T value variation of 0.8% ~ 10%. (5) The fitting curves suggest that the blood OPG concentration in Han women is the best fit to the T values of L1, L2, L3, L and Inter Tro sites, and the best fit to the T values of GT and Hip sites by the second-order polynomial fitting , And L4, Neck T values without optimal fitting curve. Conclusions The serum OPG level of Uighur elderly is lower than that of the Han nationality elderly, especially the Uyghur male is lower than that of the Han nationality. The change of blood OPG concentration may be the influencing factor of the change of T value of the elderly female.