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目的 探索更好、更方便的家兔阴茎海绵体平滑肌细胞体外培养方法。方法 分别采用组织块法、酶消化法、组织块 +酶消化法对家兔阴茎海绵体平滑肌细胞进行培养 ,在倒置显微镜下对培养过程的细胞分别作了生长情况及形态学观察。用HE染色、MTT法分别描绘原代培养细胞的生长曲线、细胞分裂指数曲线 ;用倒置显微镜观察活细胞的贴壁过程 ,计数法测定细胞贴壁率。结果 组织块法及酶消化法培养的细胞其生长状况和形态学各有自己的特点。组织块法的细胞生长慢 ,培养时间相对较长 ,纯度相对较高 ,原代培养细胞生长速度快 ,但由于该法消化时间不易掌握 ,常影响其成功率 ,多数细胞呈梭形 ,细胞的密度高 ,原代培养时间为 7～ 10d。结论 每种培养方法都有各自的优缺点。我们可根据实验的需要而选择不同的方法或联合培养
Objective To explore a better and more convenient rabbit corpus cavernosal smooth muscle cell culture in vitro. Methods The rabbit corpus cavernosum smooth muscle cells were cultured by tissue block method, enzyme digestion method and tissue block + enzyme digestion method respectively. The growth and morphology of the cultured cells were observed under an inverted microscope. The growth curve and the cell division index curve of the primary culture cells were respectively described by HE staining and MTT method. The adherent process of the living cells was observed with an inverted microscope, and the cell attachment rate was counted by a counting method. Results Tissue block method and enzyme digestion method of cell growth and morphology of their own characteristics. Tissue block method of slow cell growth, culture time is relatively long, the purity is relatively high, the primary cultured cells grow faster, but because of the law digestion time is not easy to grasp, often affect the success rate, the majority of cells were fusiform, cell High density, primary culture time is 7 ~ 10d. Conclusion Each culture has its own advantages and disadvantages. We can choose according to the experimental needs of different methods or co-culture