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目的：观察异丙嗪（ｐｒｏｍｅｔｈａｚｉｎｅ，ＰＭＺ）对下丘脑细胞内游离钙浓度（［Ｃａ２＋］ｉ）的影响。方法：以酶法制备家兔下丘脑细胞悬液，运用钙指示剂Ｆｕｒａ－２／ＡＭ作为细胞内游离钙的荧光探针测定下丘脑细胞［Ｃａ２＋］ｉ。结果：１）ＰＭＺ（０４６ｍｍｏｌ／Ｌ）使下丘脑［Ｃａ２＋］ｉ显著升高，且在一定的剂量范围内呈量效关系。２）事先向细胞悬液中加入钙通道阻滞剂维拉帕米可明显抑制ＰＭＺ诱导的［Ｃａ２＋］ｉ升高，但不能完全阻断ＰＭＺ的这种作用。结论：上述结果提示ＰＭＺ可引起下丘脑［Ｃａ２＋］ｉ升高，钙通道开放导致细胞外钙内流是ＰＭＺ引起下丘脑［Ｃａ２＋］ｉ升高的机制之一。
Objective: To observe the effect of promethazine (PMZ) on intracellular free calcium concentration in hypothalamus ([Ca2 +] i). Methods: Hypothalamic cell suspension was prepared by enzymatic method. [Ca2 +] i was measured by fluorescent probe of Fura-2 / AM, a calcium indicator for intracellular free calcium. Results: 1) PMZ (0.46 mmol / L) significantly increased [Ca2 +] i in the hypothalamus and showed a dose-response relationship within a certain dose range. 2) The addition of verapamil, a calcium channel blocker, to the cell suspension significantly inhibited PMZ-induced [Ca 2+] i elevation but did not completely block this effect of PMZ. CONCLUSION: The above results suggest that PMZ can induce the increase of [Ca2 +] i in the hypothalamus. Ca2 + influx caused by the opening of calcium channel is one of the mechanisms of PMZ-induced increase of [Ca2 +] i in the hypothalamus.