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[Objective] The research aimed to study the antibacterial action of Mentha spicata Linn extract in vitro.[Method] The cylinder-plate method was used to measure the inhibitory zone size of Mentha spicata Linn extract.The test-tube double dilution method was used to measure the minimal inhibitory concentration(MIC)of Mentha spicata Linn extract on the four kinds of animal pathogenic bacteria,which could determine the antibacterial effect.[Result] The decoction,alcohol extract and volatile oil of Mentha spicata Linn had the different antibacterial effects on the four kinds of animal pathogenic bacteria.Especially the inhibition effect on the pig staphylococcus was comparatively obvious.MIC of Mentha spicata Linn decoction I,alcohol extract III,volatile oil V and volatile oil VI were respectively 31.25,62.5,2.32 and 2.31 mg/ml.[Conclusion] The decoction,alcohol extract and volatile oil of Mentha spicata Linn had the certain antibacterial effect in vitro,and the extracts which were gained by the different extraction methods had the different inhibition effects on the experimental bacteria.
[Objective] The research aimed to study the antibacterial action of Mentha spicata Linn extract in vitro. [Method] The cylinder-plate method was used to measure the inhibitory zone size of Mentha spicata Linn extract. The test-tube double dilution method was used to measure the minimal inhibitory concentration (MIC) of Mentha spicata Linn extract on the four kinds of animal pathogenic bacteria, which could determine the antibacterial effect. [Result] The decoction, alcohol extract and volatile oil of Mentha spicata Linn had the different antibacterial effects on the four kinds of animal pathogenic bacteria. Especially the inhibition effect on the pig staphylococcus was comparatively obvious. MIC of Mentha spicata Linn decoction I, alcohol extract III, volatile oil V and volatile oil VI were respectively 31.25, 62.5, 2.32 and 2.31 mg /ml.[Conclusion] The decoction, alcohol extract and volatile oil of Mentha spicata Linn had the certain antibacterial effect in vitro, and the extracts which were gained b y the different extraction methods had the different inhibition effects on the experimental bacteria.