自1903年 Hoceheim 提出新生儿肺透明膜病(HMD)以来,对肺透明膜的主要成份和形成过程已进行了许多动物实验和临床病理学研究。Gitlin 曾提出透明膜是由血管内渗出的纤维蛋白浓缩后形成的。Natlie 利用组织化学的方法对比了 HMD 的透明膜和氧中毒小鼠肺内形成的透明膜,认为两种透明膜的化学性质基本相同,主要成份都是纤维蛋白。Hadders 等则提出透明膜是血浆浓缩后形成的。Brans 观察了氧中毒豚鼠的肺透明膜和 HMD 的透明膜认为,透明膜形成与上皮细胞坏死有关。Barter Cutz 等进一步指出透明膜的主要成份是坏死的上皮细胞,透明膜内没有纤维蛋白或者纤维蛋白很少。可见目前对透明膜的形成过程和成份等问题还存在着争论而且对其形成过程缺乏比较系统的观察。本实验利用小鼠长时间吸入高浓度氧制造透明膜,较系统的观察透明膜的形成 Since Hoceheim’s presentation of neonatal hyaline membrane disease (HMD) in 1903, many animal experiments and clinicopathological studies have been conducted on the major components of the hyaline membrane and its formation. Gitlin has proposed that the transparent membrane is formed by the concentration of fibrin exudated in the blood vessels. Natlie histochemical method used to compare the transparent membrane of HMD and oxygenated mice formed within the lung transparent membrane, that the two transparent membrane chemistry is basically the same, the main ingredient is fibrin. Hadders et al. Proposed that the transparent membrane is formed after plasma concentration. Brans looked at the transparent membranes of the hyaline membrane and the HMD of oxygenated guinea pigs and concluded that the formation of clear membrane is associated with epithelial cell necrosis. Barter Cutz et al further pointed out that the main component of the transparent membrane is necrotic epithelial cells, there is no fibrin in the transparent membrane or few fibrin. It can be seen that there are still controversies about the formation process and composition of the transparent film and the lack of systematic observation of the formation process. In this experiment, mice were exposed to high concentrations of oxygen for a long time to make transparent membranes, and the formation of transparent membranes was systematically observed