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目的:通过HPLC法测定五味子药材中的五味子醇甲、五味子酯甲、五味子甲素和五味子乙素的含量,对不同产地的五味子药材进行有效成分的对比。方法:样品采用超声波提取,采用RP-HPLC法直接测定,用十八烷基键合相,乙腈-水-冰醋酸(60∶40∶0.1)为流动相,流速1.0 mL/min,检测波长220 nm。结果:五味子醇甲、五味子酯甲、五味子甲素和五味子乙素分别在0.01306~1.3056μg(r=0.9997)、0.00265~0.2650μg(r=0.9995)、0.00204~0.2036μg(r=0.9998)和0.00765~0.7648μg(r=0.9998)范围内与峰面积具有良好线性关系。平均加样回收率分别为99.19%、99.27%、99.17%、99.28%。RSD(n=9)分别为0.95%、0.86%、0.90%和1.42%。结论:所建立方法灵敏度高、准确、简便、快速,可用于五味子药材中五味子醇甲、五味子酯甲、五味子甲素和五味子乙素的含量测定。
Objective: To determine the contents of schisandrin, schisandrin, schisandrin and schisandrin B in Schisandra chinensis by HPLC and compare the active ingredients of Schisandra chinensis from different origins. Methods: The sample was extracted by ultrasonic and directly determined by RP-HPLC with octadecyl bonding phase, acetonitrile-water-glacial acetic acid (60:40:0.1) as mobile phase, flow rate 1.0 mL/min, detection wavelength 220 Nm. RESULTS: The concentrations of schisandrin A, schisandrin A, schizandrin A and schisandrin B were 0.01306-1.3056 μg (r=0.9997), 0.00265-0.2650 μg (r=0.9995), 0.00204-0.2036 μg (r=0.9998) and 0.00765 respectively. There is a good linear relationship with peak area in the range of ~ 0.7648 μg (r = 0.9998). The average recovery rates were 99.19%, 99.27%, 99.17%, and 99.28%, respectively. The RSD (n=9) was 0.95%, 0.86%, 0.90%, and 1.42%, respectively. Conclusion: The established method is sensitive, accurate, simple and rapid. It can be used for the determination of schisandrin, schisandrin, schizandrin, and schisandrin B in Schisandra chinensis.