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目的:探讨使用组织芯片技术,观察热休克蛋白在鼻咽癌组织中的表达及临床意义。方法:以50例鼻咽癌患者鼻咽部存档石蜡标本为观察研究对象,采用组织芯片技术和免疫组织化学方法检测鼻咽癌组织中HSP 70的表达。结果:50例鼻咽癌组织中HSP70阳性表达为72%(36/50),与采用常规制片方法进行免疫组织化学染色片对比,阳性率、阳性表达强度结果基本一致,鼻咽癌中颈淋巴结转移阳性组的HSP70表达高于阴性组,差异具有显著性,P<0.01。结论:HSP70表达与鼻咽癌相关,HSP70可作为评价鼻咽癌患者预后的指标。使用自制组织芯片进行HSP70免疫组织化学染色分析,效果良好,显示了组织芯片技术应用于临床病理免疫组织化学染色和实验分析的良好前景。
Objective: To investigate the expression and clinical significance of heat shock protein in nasopharyngeal carcinoma using tissue microarray. Methods: The specimens of nasopharyngeal paraffin embedded in 50 cases of nasopharyngeal carcinoma were used as the observation objects. The expression of HSP70 in nasopharyngeal carcinoma tissues was detected by tissue microarray and immunohistochemistry. Results: The positive expression of HSP70 in 50 cases of nasopharyngeal carcinoma was 72% (36/50). Compared with the immunohistochemical staining with routine method, the positive rate and the positive expression intensity were basically consistent. The expression of HSP70 in lymph node positive group was higher than that in negative group, the difference was significant (P <0.01). Conclusion: HSP70 expression is associated with nasopharyngeal carcinoma, and HSP70 may be used as an index to evaluate the prognosis of patients with nasopharyngeal carcinoma. The results of immunohistochemical staining of HSP70 using homemade tissue microarray showed good results, showing a good prospect of tissue microarray technology for clinicopathological immunohistochemical staining and experimental analysis.