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目的 了解大鼠感觉神经是否具有２９ Ｋ Ｄａ（ 分子量为２９ ０００ 道尔顿） 蛋白。方法 提取大鼠脊神经节及背根纤维作为感觉神经组织材料，腹根纤维作为运动神经组织材料。分别对两种材料进行匀浆、离心、电泳，并将感觉神经组织材料匀浆上清液进行 Ｄ Ｅ Ａ Ｅ－ Ｓｅｐｈａｃｅｌ 阴离子交换层析，浓缩及 Ｈ Ｐ Ｌ Ｃ 凝胶过滤层析。结果 （１） 电泳感觉神经上清液有２９ Ｋ Ｄａ 蛋白条带。（２） 感觉神经上清液经过 Ｄ Ｅ Ａ Ｅ Ｓｅｐｈａｃｅｌ 阴离子交换层析获较好的纯度。（３） 将含有２９ Ｋ Ｄａ 组份进行 Ｈ Ｐ Ｌ Ｃ 凝胶过滤层析２９ Ｋ Ｄａ 蛋白带纯度达８０ ％ 以上。结论 （１） 大鼠脊神经节及背根纤维中含有感觉神经特异蛋白２９ Ｋ Ｄａ 并可得到分离纯化，而腹根纤维中不含此特异蛋白。（２） 建立了具有重复性的大鼠２９ Ｋ Ｄａ 蛋白的纯化方法。
Objective To determine if the rat sensory nerve has a 29 KDa (29,000 Dalton) protein. Methods Spinal ganglion and dorsal root ganglion fibers were extracted from the rats as sensory nerve tissue materials, and as the motor nerve tissue material. The two materials were respectively homogenized, centrifuged and electrophoresed. The sensory nerve tissue homogenate supernatant was subjected to D E A-Sephacel anion exchange chromatography, concentration and H P L C gel filtration chromatography. Results (1) The electrophoresis sensory neuron supernatant has a 29 K Da protein band. (2) Sensory neuron supernatants passed DEAE Sephacel anion exchange chromatography to obtain better purity. (3) The 29 K Da fraction containing 29 K Da is subjected to H P L C gel filtration chromatography with a purity of over 80% for the 29 K Da protein band. Conclusion (1) The rat spinal ganglion and dorsal root fibers contain sensory nerve specific protein 29 K Da and can be isolated and purified, while the abdomen fiber does not contain this specific protein. (2) The method of purification of rat 29 K Da protein was established.