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提出了一种利用高效液相色谱法同时分析产琥珀酸放线杆菌发酵液中葡萄糖和有机酸的方法。在EclipseXDB C8(150mm×Φ4.6mm,5μm)色谱柱上,以5mmol LH2SO4溶液(pH2.5)作流动相,流速为1mL min,采用示差折光检测器,一次进样可同时定性及定量分析待测试样中的有机酸及葡萄糖,每一样品的分析时间不超过9min。确定了测定各物质的工作曲线的回归方程、线性范围、相关系数和检出限。测定发酵液中葡萄糖、琥珀酸和副产物乳酸的相对标准偏差在0.41%～0.89%范围内,平均回收率在99.6%～100.8%之间。
A method for the simultaneous analysis of glucose and organic acids in the fermentation broth of Actinobacillus succinogenes by high performance liquid chromatography was proposed. In EclipseXDB C8 (150mm × Φ4.6mm, 5μm) column with 5mmol LH2SO4 solution (pH2.5) as the mobile phase, the flow rate of 1mL min, using a refractive index detector, a single injection can be qualitative and quantitative analysis to be Test samples of organic acids and glucose, each sample analysis time does not exceed 9min. The regression equation, the linear range, the correlation coefficient and the detection limit of the working curve for each substance were determined. The relative standard deviations (RSDs) of glucose, succinate and byproduct lactic acid in the fermentation broth ranged from 0.41% to 0.89%, and the average recoveries ranged from 99.6% to 100.8%.