论文部分内容阅读
目的建立大鼠的急性酒精性肝损伤模型,研究体内白藜芦醇对急性酒精性肝损伤的保护作用,同时初步探讨其作用机制。方法采用完全随机法将SD大鼠分为6组:空白对照组,模型组,白藜芦醇高(100 mg/kg)、中(50 mg/kg)、低(25 mg/kg)剂量组及阳性对照组。除空白组外其他组大鼠每日以55度红星二锅头灌胃,每次7.5 ml/kg,2次/日,空白组大鼠则每日以等容剂的生理盐水灌胃。另外,白藜芦醇高、中、低剂量组及阳性对照组大鼠每日以相应药物灌胃给药,1次/日;空白组及模型组大鼠则以等容剂的1%的羧甲基纤维素钠灌胃。连续灌胃7 d。第8天取大鼠血液及肝脏,检测血清及肝组织匀浆中的谷丙转氨酶(ALT)、谷草转氨酶(AST)、超氧化物歧化酶(SOD)及丙二醛(MDA)。同时检测血清中白介素-1β(IL-1β)和肿瘤坏死因子-α(TNF-α)的水平,另取部分肝脏组织做病理切片。结果与空白对照组比较,模型组的血清及肝匀浆中的ALT、AST活力及MDA含量显著升高,且SOD活力显著降低;白藜芦醇高、中、低剂量组较模型组ALT、AST活力及MDA含量显著降低,SOD活力显著提高;白藜芦醇显著降低急性酒精性肝损伤大鼠血清中IL-1β和TNF-α水平的升高。病理学检查显示白藜芦醇治疗组能改善模型组大鼠细胞水样变性及脂肪样变性。结论白藜芦醇对大鼠急性酒精性肝损伤有保护作用,其机制可能与清除氧自由基、抗脂质过氧化损伤、抑制炎症反应水平有关。
Objective To establish a rat model of acute alcoholic liver injury and to study the protective effect of resveratrol on acute alcoholic liver injury in rats and its possible mechanism. Methods The SD rats were randomly divided into 6 groups: blank control group, model group, high dose of resveratrol (100 mg / kg), middle dose (50 mg / kg) and low dose And positive control group. Except the blank group, rats in other groups were orally gavaged at 55 degrees Red Star Erguotou every day for 7.5 times a day for 2 times a day. Rats in the blank group were given normal saline with isotonic agent daily. In addition, resveratrol high, medium and low dose groups and the positive control group of rats daily administration of the corresponding drug gavage, 1 / day; blank group and model group rats were treated with isotonic agent 1% Carboxymethyl cellulose sodium gavage. Continuous gavage 7 d. On the 8th day, the blood and liver of the rats were taken out. ALT, AST, MDA and malondialdehyde (MDA) in serum and liver homogenate were measured. Serum levels of interleukin-1β (IL-1β) and tumor necrosis factor-α (TNF-α) were measured at the same time. Some liver tissues were taken for pathological examination. Results Compared with the blank control group, the activities of ALT, AST and MDA in the serum and liver homogenate of the model group increased significantly and the activity of SOD decreased significantly. Compared with the model group, the levels of ALT, AST activity and MDA content decreased significantly, SOD activity increased significantly; resveratrol significantly reduced serum levels of IL-1β and TNF-α in rats with acute alcoholic liver injury. Pathological examination showed that the resveratrol treatment group could improve the watery degeneration and fatty degeneration of the model rats. Conclusion Resveratrol has a protective effect on acute alcoholic liver injury in rats. The mechanism may be related to scavenging oxygen free radicals, anti-lipid peroxidation injury and inhibiting inflammatory reaction.