4-喹诺酮类抗生素在治疗许多软组织感染包括细菌性前列腺炎中有重要价值。临床研究中为了测定血清和前列腺的氟啶酸浓度,建立了一个简单易行的、用HPLC测定氟啶酸的直接测定方法。此方法能把氟啶酸和其主要代谢物氧化氟啶酸分离开,并且适用于分析其他的4-喹诺酮。本次实验所用的固定相为Microbonda-pak C18(Waters Z-Module),流动相为含15～25％甲醇的0.1M柠檬酸(甲醇的实际最 4-Quinolone antibiotics are of great value in the treatment of many soft-tissue infections, including bacterial prostatitis. In clinical studies, a simple and straightforward method for the determination of fiuidinic acid in serum and prostate by HPLC was established. This method separates oxypicidin from its major metabolite oxyfurfural and is suitable for the analysis of other 4-quinolones. The stationary phase used in this experiment was Microbonda-pak C18 (Waters Z-Module) with a mobile phase of 0.1 M citric acid containing 15-25% methanol (the actual