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目的研究人类白细胞相关抗原-G(HLA-G)及其各个亚型mRNA在正常妊娠及重度子前期患者胎盘组织中的表达。方法选取2005-01-2005-06第四军医大学唐都医院10例重度子前期患者(研究组)及10例正常妊娠胎盘组织(对照组),应用荧光定量RT-PCR比较两组间HLA-G及其各个亚型mRNA(HLA-G1、G2、G3、G4、G5、G6)的表达。结果与对照组相比,重度子前期患者胎盘组织中总HLA-GmRNA显著降低,以HLA-G1、HLA-G3降低为主,差异有统计学意义(P<0.05)。结论HLA-G1与HLA-G3在胎盘组织中的低表达可能与妊娠期高血压疾病的发病和病理生理过程有关。
Objective To study the expression of human leukocyte associated antigen-G (HLA-G) and its subtypes mRNA in placenta of normal pregnancy and patients with severe preeclampsia. Methods Ten patients with severe preeclampsia (study group) and 10 normal pregnancy placenta tissues (control group) from Tangdu Hospital of the Fourth Military Medical University from January 2005 to June 2005 were enrolled. Fluorescent quantitative RT-PCR was used to compare HLA- -G and its subtypes mRNA (HLA-G1, G2, G3, G4, G5, G6) expression. Results Compared with the control group, the total HLA-G mRNA in the placenta of patients with severe preeclampsia decreased significantly, with the decrease of HLA-G1 and HLA-G3, with significant difference (P <0.05). Conclusion The low expression of HLA-G1 and HLA-G3 in placenta may be related to the pathogenesis and pathophysiological process of hypertensive disorders in pregnancy.