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目的 :检测胃癌和相应癌旁组织中 u PA和 u PAR m RNA表达水平 ,并探讨它们与胃癌的侵袭、转移之间的关系。方法 :运用 c DNA- m RNA Northern印迹杂交方法定性和定量地检测了 2 0例胃癌和相应癌旁组织中u PA和 u PAR m RNA的表达水平。结果 :在检测的 2 0例胃癌组织中分别有 13例 u PA和 15例 u PAR m RNA表达显著高于相应的癌旁组织 ( P<0 .0 1) ,其中 12例 u PA和 u PAR m RNA协同高于相应癌旁组织 ;11例伴有淋巴结转移的胃癌组织中 u PA和 u PAR m RNA表达水平显著高于 9例无淋巴结转移者。结论 :u PA、u PAR m RNA在胃癌组织中表达增强 ,并且与胃癌侵袭、转移密切相关
Objective : To detect the expression of u PA and u PAR m RNA in gastric cancer and corresponding adjacent tissues, and to explore their relationship with the invasion and metastasis of gastric cancer. METHODS: The expression levels of u PA and u PAR m RNA in 20 cases of gastric cancer and corresponding adjacent tissues were qualitatively and quantitatively detected by c-DNA m RNA Northern blot hybridization. RESULTS: Twenty-three cases of u PA and 15 cases of u PAR mRNA expression were significantly higher in paraffin-embedded tissues (P < 0.01), of which 12 cases were u PA and u PAR. The mRNA level of m PA and u PAR mRNA in 11 gastric cancer tissues with lymph node metastasis was significantly higher than that in 9 patients without lymph node metastasis. Conclusion : u PA and u PAR m RNA expression is enhanced in gastric cancer tissues and is closely related to the invasion and metastasis of gastric cancer.