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测定了Ｍ－ＣＳＦ，Ｃ－ＣＳＦ，ＧＭ－ＣＳＦ，ＩＬ－３，ＩＬ－６，ＭＧＦ，ＬＩＦ和ＴＧＦ－β＿１８种重组人生长因子单个或不同组合对白血病细胞系Ｊ６－１，Ｊ６－２集落形成率的影响。单个因子除ＴＧＦ－β＿１和ＬＩＦ表现出明显抑制效应外，其它因子均有不同程度的刺激活性。而在双因子协同下，ＴＧＦ－β＿１除与Ｍ－ＣＳＦ协同外，仍表现出明显的抑制作用，表明ＴＧＦ－β是Ｊ６－１，Ｊ６－２细胞系重要的负调节因子。从三种因子组合结果看出Ｍ－ＣＳＦ是Ｊ６－１细胞必需的细胞因子。值得注意的是，ＴＧＦ－β＿１与Ｍ－ＣＳＦ的组合对Ｊ６－１细胞有很强的增殖刺激效应。另外由Ｊ６－１细胞膜分离出的膜相关细胞因子（ＭＡＦ－Ｊ６－１）单独和与ＴＧＦ－β＿１联合，对Ｊ６－１，Ｊ６－２细胞的作用与Ｍ－ＣＳＦ的作用相似
The leukemic cell lines J6-1 and J6-2 were determined by single or different combinations of M-CSF, C-CSF, GM-CSF, IL-3, IL-6, MGF, LIF and TGF-β18 recombinant human growth factors Effect of formation rate. In addition to TGF-β_1 and LIF, a single factor showed significant inhibitory effect, other factors have different levels of stimulating activity. However, in addition to synergism with M-CSF, TGF-β 1 showed a significant inhibitory effect under the synergistic effect of two factors, indicating that TGF-β is an important negative regulator of J6-1 and J6-2 cell lines. From the results of the three factor combinations, M-CSF is an essential cytokine for J6-1 cells. It is noteworthy that the combination of TGF-β 1 and M-CSF has a strong proliferative stimulatory effect on J6-1 cells. In addition, membrane-associated cytokines (MAF-J6-1) isolated from the J6-1 cell membrane, alone and in combination with TGF-β_1, function similarly to M-CSF for J6-1 and J6-2 cells